Research question- How do the different concentrations of murphy infusion affect the enzyme activity?
- Hypothesis– If the concentration of the murphy infusion is higher, the clip taken for the filter paper to float of the H peroxide solution, will be higher. This is because, at low murphy infusion concentration, the rate of enzyme activity, there are less hits between the substrate and the active site where as if the murphy extract concentration is higher, there are more hits between the active site and the substrate.
- Variables- Mugwump– the independent variable for this experiment is the concentration of the murphy infusion which will be varied by altering the volume of the murphy infusion by adding different volumes of distilled H2O and diminishing the volume of the murphy infusion in each reading.
- Controlled variable– the controlled variable for this experiment is the concentration of the Hydrogen Peroxide ( H2O2 ) in which is 10 % and he volume taken for each reading is besides kept the same in which is 5cm3. The entire volume, of the murphy infusion and the distilled H2O will be maintained at 10 centimeter 3.
- Dependent variable– The dependant variable for this experiment is the clip taken for the piece of filter paper to lift to the top of the H peroxide solution in the beaker.
- 3 Panpipes of a entire volume of 10 centimeters 3 each.
- Test tubing
- Potato infusion of a volume of 150cm3 and a concentration of 100 %
- Distilled H2O of a volume of 150cm3
- Beakers ( 250 cm3, 50 cm3 )
- Filter paper cut into really little pieces.
- Hydrogen Peroxide ( H2O2 ) of a volume of 150 cm3 and a concentration of 10 % .
- Pour the murphy infusion in a beaker for all the tests.
- Pour distilled H2O into another beaker for all the tests.
- Pour H peroxide into another beaker for all the tests.
- Take one syringe, and step 10 cm3 of murphy infusion and force out it into the little beaker. Take another little beaker and utilize the other syringe to take 10 cm3 of H2O2 and pour it into another little beaker.
- Take the pincer to pick up the minute piece of paper and dip it into the 100 % concentrated murphy infusion until its wet plenty.
- Drop it in into the H peroxide solution and get down the stop watch. The stop ticker should be stopped when the piece of filter paper floats.
- Throw the murphy infusion and the H peroxide solution and rinse the beakers
- Record the clip taken
- Since the entire volume of the concentrated murphy infusion and the diluted murphy infusion has to be 10 milliliter, repetition stairss 5 to 8 for the concentrations of 75 % , 50 % , 30 % ,25 % , and 10 % of the infusion.
- Stairss 1 to 8 should be performed for the 2nd and the 3rd.
Consequences of the experiment:
My hypothesis was right since my consequences show that because if the concentration is higher, the clip taken for the piece of filter paper to drift will be less. And if the concentration decreases, the clip taken additions it is shown in the curve on the graph since the volume is diminishing the clip taken is increasing.
There might hold been some mistakes which could hold caused minor fluctuations in the readings. The syringe used to take the murphy infusion out of the beaker, is hard to take the readings so at that place might hold been a difference in ( +0.1cm3 ) in the volume used. This might hold resulted in the alteration in the concentration of the murphy infusion doing the clip taken for the filter paper to drift to besides fluctuate by ( +1 sec ) .
The defects in the process, could hold been due to the use of the syringe in which was in mensurating the sum of the murphy infusion and the distilled H2O. There were no other mistakes which have caused a faulty reading.
All of the other of import variables were controlled to avoid mistakes every bit much as possible as it has been shown in the proceedure.
took adequate readings for the norm to acquire the best possible consequences so there was no deficiency in the reproduction.
Improvements to be made:
Some betterments can be made by rinsing the tools used more frequently. More reproductions of the consequences should hold been taken to plot a better curve on the graph. The readings on the syringe should hold been taken more exactly.